Electron Crystallography Workshop 2010

The workshop on membrane protein 2D crystallization and electron crystallography is aimed at everybody (PhD students, PostDocs, group leaders, etc.), who is interested in the structure determination of membrane proteins (or other 2D protein crystal arrays) by electron crystallography.

Videos

https://www.youtube.com/playlist?list=PLteVxMX-rlQvxPA4SkvFOT-j4cB0yCIpG

Aims

This workshop has two major aims:

Advancing the technology: To bring together many of the leading groups in electron crystallography to forge innovative collaborations for new technology development.

Training: To provide a unique forum to train skilled biochemists and electron microscopists (PhD, Post docs and beyond), in membrane protein 2D crystallization, electron crystallographic data collection and data processing.

Membrane protein solubilisation and crystallization
Sample preparation for electron microscopy
Cryo-EM data collection (imaging and electron diffraction)
Data processing with the MRC, IPLT and 2dx software packages
Data evaluation and model building

Program

The workshop will feature lectures in the morning, practicals in the afternoon, and science talks in the evening. Membrane protein crystallization trials will introduce lipid monolayer crystallization methods, or 2D crystallization with dialysis devices, or with a cyclodextrin robot. We will demonstrate automated 2D crystal screening, and present cryo-EM imaging and electron diffraction on the CM200FEG and Titan Krios. We will lecture image processing using MRC, IPLT and 2DX software systems. Computer practicals will use machines in a local computer room. However, students are also invited to bring their own laptop computer (Linux or OSX), where we can try to install the image processing software packages and run the processing on your own computers during the workshop.

Workshop Lectures: August 2-6, 2010 (Monday-Friday) Arrival: Sunday, August 1, 2010. Departure: Saturday, August 7, 2010

The workshop will be divided into three blocks: Lectures (morning), hands-on practicals (afternoon), and research talks (evening).

2010-workshop-program.pdf

Karen Bergmann, University of Basel, Switzerland. Phone: +41 61 387 32 31

Thomas Walz, Harvard Medical School, Boston, MA, USA.

Andreas Engel, University of Basel, Switzerland.

Henning Stahlberg, University of Basel, Switzerland.

C-CINA (Centre for Cellular Imaging and Nano Analytics) Biozentrum, University Basel WRO-1058 Mattenstrasse 26 CH-4058 Basel Switzerland