Minmax
The minimum and maximum grey values for the images.
Minimum grey value, and maximum grey value
The minimum and maximum grey values specify the upper and lower cut-offs for the images. A micrograph that was digitized on a scanner sometimes has dust pixels that are completely out of range with respect to the majority of the pixels in the image. The same holds for CCD camera images that have hot pixels or dead pixels or suffers from “cosmic events” or X-rays. The MIN and MAX limitations are then used to threshold the pixels to values between these two upper and lower boundaries.
For a typical BYTE format image (0…255 as grey values), these numbers can be set to 10 and 245. They can, however, also be set to 0 and 255, which then would leave the images as they are, without any thresholding.
Crop Histogram to reduce Over- and Under-flows? [crop_histogram]
This switch allows you to decide if your input image after conversion from TIFF to MRC file format should be limited in its pixel values. If so, the following two parameters crop_histogram_percent and crop_histogram_stdev would be used to decide on the applied limits.
crop_histogram_percent [crop_histogram_percent]
This parameter defines by how many percent of its original width (MAX-MIN) the histogram should be cropped on both ends. If your histogram goes from 0 to 100, and you set this value to 5, then after cropping your pixel values will be limited to the range between 5 and 95.
crop_histogram_stdev [crop_histogram_stdev]
This parameter defines, how many times the STanDardDeviation the histogram width should be maximally. If you histogram originally goes from 0 to 100, with a mean of 60 and a standard deviation of 10, and you set this value to 3, then after cropping your image will have pixels in the range between 30 and 90.
Generate periodogram? [generatePeriodogram]
See Periodogram